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в II ̳ - internet- 21-23 2016 . , Tokaj wines as source of polyphenols with positive effects to health Ballov udmila1, Zuzana Eftimov2, Slavomr Kurhajec1, Eftimov Jarmila1 The University of Veterinary Medicine and Pharmacy in Koice, Deparment of Pharmacognosy and Botany, Komenskho 73, 041 81 Koice, Slovakia Slovak University of Agriculture in Nitra, Faculty of Agrobiology and Food Resources, Trieda A. Hlinku 2, 949 76 Nitra, Slovakia Tokaj is the region in the southeastern Slovakia and also in the northeastern Hungary. Tokaj wine are appreciated for their unique aroma and delicious taste. Three types of wine grapes are registered on this region, namely Furmint, Lipovina and Mukt lt. Many different types of wines are produces by special vinification techniques from these types. Tokajske putnove wines, so called botrytized wine, are produced from the grapes infected by noble rot Botrytis cinerea, which presence has a positive influence on quality of certain wines. The results of many epidemiological studies have shown that moderate wine consumption has a positive effect to human health, for example to prevention and therapy of cancer, Alzheimers disease, coronary heart disease, etc.

Polyphenols obtained in Tokaj wine are one of the main secondary metabolites responsible for their antioxidant, anti-inflammatory or anti-aging activity (Stratil, 2008).

In the present study was measured the amount of polyphenols by Folin-Ciocalteu method (Folin 1927; Singleton 1965). Two different assays were used to the evaluated antioxidant activity (DPPH and PRAC method) in different wine samples from wine cellar in Tokaj region in Slovakia. Botrytized wine Esencia, Tokajske 6-putnove, Tokajske 5putnove, Tokajske 4-putnove, Tokajske 3-putnove, and non-botrytized wine Furmint, Furmint Barrique, Mukt lt, Lipovina, Samorod sladk, Samorod such and Fordit were studied (Brand-Williams 1995; Cacig 2006).

The results of our experiments have shown that the 6-, 5-,4- putnove wine comprise more polyphenols than the non-botrytized wine. In non-botrytized wines the highest level of flavonoids, phenolic acids, tannins and anthocyanins (which belong to polyphenols) were found in Samorod sladk and Samorod sladk. Content of polyphenols in other samples is qualified in this decreasing order Mukt Furmint Fordit Svt Urban Lipovina Furmint Barrique. The vinification techniques, climate, intensity of solar irradiation at the ripening time of grapes, fungus pressure have a great influence on secondary metabolites level in wine (Stratil, 2006). The results of both methods for the determination of antioxidant activity were similar. Moreover, they were in correlation with the results of polyphenolic content.

The highest antioxidant activity and also amount of polyphenols were observed in 6-putnove wine. Similarly higher antioxidant activity was observed in botrytized wine that in non-botrytized. Values of PRAC and DPPH activity are quantified in this order form highest to lowest activity: Tokajske 6-putnove, Tokajske 5-putnove, Tokajske 4-putnove, Tokajske 3-putnove, Samorod sladk, Mukt lt, Fordit, Svt Urban, Furmint, Lipovina, Furmint Barrique and Samorod such.

Our investigation has clearly shown that Tokaj wines are a great source of polyphenolic compounds and other species with high antioxidant capacity. The results has also shown that antioxidant activity of Tokaj wines depend on the level of polyphenols.

This study further supports the fact that the consumption of wine in small amount seems to be beneficial for human health especially for cardiovascular diseases.

References Brand-Williams, W., et. al. (1995). Use of a free radical method to evaluate antioxidant activity. Lebensm. Wiss. In: Technol., 28, 25-30.

Cacig, S. I., et al. (2006). Spectrophotometric method for the study of the antioxidant activity applied on Ziziphus jujoba and Hydrangea paniculata aqueous extracts. In: Matica Srpska Proceedings for Natural Sciences, 8793.

Folin, O. Tyrosine and tryptophan determinations in proteins. J. Biol. Chem.

1927, 73, 672-649.

Singleton, V. L.; Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. In: Am. J. Enol. Vitic. 16, 144-158.

Stratil, P., et al. (2008). Comparison of the phenolic content and total antioxidant activity in wines as determined by spectrophotometric methods. In: Czech Journal of Food Science, 26 (4), 242-253.

   

Plantago media L., hoary plantain, Plantaginaceae, is considered a medicinal plant, but to a lesser extent than the related species - P.lanceolata and P.major. It was reported to contain iridoids and one phenylethanoid, which are typical secondary metabolites of the genus Plantago L. [Rnsted et al. 2003]. In vitro propagation of P.media was performed for the first time at our department [Budzianowska et al. 2015].

The objective of the present work was an analysis of compounds in the plants of P.media from in vitro cultures. Plant material from in vitro propagation on four variants of Murashige-Skoog medium served for the preparation of methanol extracts separately from the dried shoots and the roots (eight extracts). From each extract chloroform and water fractions were obtained. Thin-layer chromatography of those fractions revealed different pattern of compounds in the roots and shoots, which was not influenced by the kind of medium. The water fractions from the shoots and roots, from the same medium, were separated by a column chromatography into iridoid and phenylethanoid fractions.

The phenylethanoid fraction from the shoots was further separated by column and preparative thin-layer chromatography to give four compounds, of which three were identified as acteoside (verbascoside), plantamajoside and martinoside by cochromatography with reference substances and spectral UV analyses with diagnostic reagents. The content of acteoside and plantamajoside was determined by a TLC densitometry. The shoots were found to contain more acteoside than plantamajoside, while the plants from natural habitats were reported to contain significantly more plantamajoside than acteoside [Olennikov et al. 2011]. In turn, in the roots from in vitro propagation the content of plantamajoside was higher than that of acteoside.

Literature Budzianowska A., Makiewicz M., Budzianowski J. In vitro regeneration of Plantago

media L. from shoot tips and other seedling explants. Biotechnologia, 2015; 96(1):

123.

Olennikov, D.N., Tankhaeva, L.M., Stolbikova, A.V., Petrov, E.V. 2011.

Phenypropanoids and polysaccharides from Plantago depressa and P. media growing in Buryatia. Chem. Nat. Compd. 47, 165169.

Rnsted N., Franzyk H., Mlgaard P., Jaroszewski J.W., Jensen S.R. Chemotaxonomy and evolution of Plantago L. Plant. Syst. Evol. 242: 63-82 2003.

   

Plantago ovata Forssk. (Plantaginaceae) is native to flora of Asia. It is cultivated for its seeds and seed husk, which are commercially important mild laxative remedies due to the high content of a mucilage. The plants were found to contain iridoids and phenylethanoids, which are metabolites characteristic for the genus Plantago L. [Rnsted et al 2003]. In our previous investigations shoot and callus cultures of P.ovata were established and they were found to produce phenylethanoid compounds [Budzianowska and Budzianowski 2009], The aim of the present work was a detailed analysis of phenolic compounds in three types of callus of P.ovata.

The occurence of phenolic compounds was analysed in callus cultures induced from different explants, like leaves, roots and hypocotyls. The methanol extracts were prepared from each callus type and separated by column chromatography on polyamide into water, methanol, methanol-0.1% ammonia and methanol-0.1% formic acid fractions.

Chromatographic examination of those fractions by thin-layer chromatography (TLC, HPTLC) showed the presence of phenolic compounds only in the methanol fractions and their composition was similar for all types of callus. The methanol fraction from the callus derived from leaves was separated by preparative thin-layer chromatography on polyamide to give four compounds. They were identified as phenylethanoid glucosides acteoside (verbascoside) and plantamajoside as the main constituents and probably martinoside and leucosceptoside A as minor compounds by co-chromatography with reference compounds and UV spectral analysis with diagnostic reagents.

Literature Budzianowska A., Budzianowski J. Phenolic compounds in shoot and callus cultures of Plantago ovata Forssk. Acta. Biolog. Cracov. ser. Bot. 51 suppl. 1, 34, 2009.

Rnsted N., Franzyk H., Mlgaard P., Jaroszewski J.W., Jensen S.R. Chemotaxonomy and evolution of Plantago L. Plant. Syst. Evol. 242: 63-82, 2003.

   

About 7 variety of plants belong to Rosaceae family were collected from plants growing in Botanical Garden of Poland (Cotoneaster bullatus, Cotoneaster dielsianus, Cotoneaster francheti, Cotoneaster melanocarpus, Cotoneaster moupinensis, Cotoneaster simonsii, Cotoneaster uniflorus) in August, 2014 year. The seeds were cleaned in air screen cleaner to remove immature and broken seeds, dried by air condition.

The seeds were stored in paper bags at +4C temperature.



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