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ϲ ղ ϲϲ̲IJͲ. 612.111: 612.393 ϲ ղ ϲϲ̲IJͲ Բ-ղ̲Ͳ ˲ ...

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IN VITRO

. . 1, . . 1, . . 2, . . 3

. . 3, . . 4, . . 1

. , 4, 79005,

, . , 23, 79005, , . 40 , 27, 73002, . , 69, 79010, (RHb, HbO2, HbCO, SHb, MetHb), , CNMetHb-Coomassi G-250 .

, 䳿 HbO2. HbO2 . HbO2, MetHb, CNMetHb CNMetHb-Coomassi G-250.

䳿 . ij .

: , , , , , , .

³, (, , ) , , , 㳿, . 䳿 / Studia Biologica 2009 3/2 . 2334 24 . . , . . , . . , . . , . . , . . , . . a , , , . - , , , . , , , . - , .

³, , , , , . , , .

, . . , . , , , . , .

, , 䳿 [8, 17]. , 䳿 , (, ó, , ). , 䳿 [5, 16, 18].

, , , - (O2) [9]. , 䳿 2 2 . , Fe2+ Fe3+ [3, 6]. , , . .

, , , .

, , - .

, - , .

䳿 / Studia Biologica 2009 3/2 . 2334 ϲ ղ ϲϲ̲IJͲ...

(3 000 / ) [10, 14]. . , [10]. , . in vitro 1, 2 3 , , , .

в ˲

, 쳿 . :

, ( 1), : 1,6--4--1,2,3,4,5,7--[3,4-d]--2,5(1) ( 2) (1,6--4-(2-)-1,2,3,4,5,7--[3,4-d]--2,5(1) ( 3) [10, 14].

, . . 500 g NaCl (0,150 ). 5 500 g. [20]. [13]. 5 : -, -, -, - (RHb, HbO2, COHb, SHb, MetHb) [1, 19]. dz [15], [11]. , (CNMetHb). 1, 2, 3 1:3. : 1 1 /;




140 .


2 1 /; 3 0,5 /. ϳ 1 , , . . . () Coomassi G-250 Fluca (). : 2 CNMetHb (50 ) 2 Coomassi G-250 (50 ). 10 Specord M-40 450750 .

.

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2. , , , 䳿 / Studia Biologica 2009 3/2 . 2334 26 . . , . . , . . , . . , . . , . . , . . a . 䳿 , , 1, 2 3 - .

㳿, , , HbO2 9497% [1, 2, 4,16, 2123]. , , (. 1). , 䳿 1. 1,5%. , , , [13]. 2 3 , 94,16%. - .

1. 䳿 ( 1) ( 2 3) in vitr, (Mm, n = 610), % The amount of hemoglobin ligand forms under the effect of Spyrocarbon Table 1.

(substance N1) and pirolopirymidyndion derivatives (substances N2, N3) in vitro, (Mm, n = 610), % ˳ RHb HbO2 COHb SHb MetHb 1 0,460,03 94,180,42 4,270,48 0,730,42 0,360,15 () (n=10) (+ 1) 0,790,01 92,051,19 4,630,77 0,960,45 1,480,58 (n=8) 3 (+ 2) 0,100,01 94,161,17 5,020,64 0,130,07 0,690,07 (n=6) 4 (+ 3) 0,100,01 94,450,35 4,550,84 0,440,25 0,560,06 (n=6) . : -, -, (HbO2, MetHb, CNMetHb), Coomassi G-250 () 䳿 1, 2, 3 450750 . . 14 . 2.

, 1 HbO2 (. 1).

. 2 CNMetHb MetHb 1 䳿 / Studia Biologica 2009 3/2 . 2334 ϲ ղ ϲϲ̲IJͲ...

,..

   

.1. (HbO2 ) in vitro:

1 䳿 ; 541,7 576,6 ;

2 䳿 ; 541, 7 576,6

Fig. 1. Oxyhemoglobin (HbO2 ) electron spectra of healthy donors blood in vitro:

1 without spyrocarbon; maxima 541,7nm, 576,6 nm;

2 incubated with spyrocarbon; maxima 541, 7nm, 576,6 nm,..

   

. 2. 1 () in vitro:

1 CNMetHb; 542,2 ;

2 CNMetHb + c; 541,1 ;

3 MetHb; 501 631,2 ;

4 MetHb + c - 499,0 630,4 Fig. 2. Hemoglobin ligand forms electron spectra of healthy donors blood under the effect of substance N1 (Spyrocarbon) in vitro:

1 CNMetHb; absorption maxima 542,2 nm;

2 CNMetHb + spyrocarbon; absorption maxima 541,1 nm;

3 MetHb; absorption maxima 501 631,2 nm;

4 MetHb + spyrocarbon; absorption maxima 499,0 i 630,4 nm 䳿 / Studia Biologica 2009 3/2 . 2334 28 . . , . . , . . , . . , . . , . . , . . a 2. ( 1), ( 2, 3),, Characteristic parameters of healthy donors blood hemoglobin ligand form Table 2.

electron spectra under the effect of Spyrocarbon (substance N1), pirolopirymidyndion derivatives (substance N2, N3),,

   



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