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ò 2 03.2009 1 (117) 2015 , 12015. 06:631/635 , , ...

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6. : , / . , . ϳ, . , . // . 2014. 1. . 9698.

7. . . . 㳿 : . . / . . , . . . 4- ., ., . : . 㳿, 2014. 1040 .

8. : , / . , . ϳ, . , . // . 2013. 12. . 88-90; 2014. 1. . 96-98.

9. . . / .. . 5- ., . . .: , 1985. 351 .

10. 쳿/ . . , . . , . . , . . ; .

. . . .: ij, 2005. 288 .

11. 㳿 / [ . ., . ., . ., . .] : . ., 2000. 203 .

12. . . 쳿: / . . , . . . . : ., 1994. 334 .

REFERENCES

1. Burjany ta kontroljuvannja i'h chysel'nosti v agrocenozah / M. Ja. Bomba, M. I. Bomba, G. T. Perig, V. Pohodenko // Agronom. 2009. 1. S. 3840.

2. Borona V. P. Integrovana systema zahystu posiviv soi' vid burjaniv / V. P. Borona, V. S. Zadorozhnyj, M. V. Pervachuk // Vyrobnyctvo, pererobka i vykorystannja soi' na kormovi ta harchovi cili: materialy Vseukr. konf., 3 serp. 2000 r. Vinnycja, 2000. S. 6566.

3. Storchous I. Porazka burjaniv na sojevomu poli / I. Storchous // Agrobiznes S'ogodni. 2012. 12. S. 4247.

4. Sergijenko V. Hvoroby soi' ta zahody i'h obmezhennja / V. Sergijenko // Agrobiznes S'ogodni. 2012. 11. S. 1823.

5. Markov I. Diagnostyka infekcijnyh hvorob soi' / I. Markov // Agrobiznes S'ogodni. 2013. 12. S. 2028.

6. Hvoroby soi': diagnostyka, osoblyvosti rozvytku ta zahody zahystu / M. Kyryk, M. Pikovs'kyj, Ju. Taranuho, S. Lych // Propozycija. 2014. 1. S. 9698.

7. Petrychenko V. F. Roslynnyctvo. Tehnologii' vyroshhuvannja sil's'kogospodars'kyh kul'tur: navch. posib. / V. F. Petrychenko, V. V. Lyhochvor. 4-e vyd., vyprav., dopov. L'viv : Ukr. tehnologii', 2014. 1040 s.

8. Hvoroby soi': diagnostyka, osoblyvosti rozvytku ta zahody zahystu / M. Kyryk, M. Pikovs'kyj, Ju. Taranuho, S. Lych // Propozycija. 2013. 12. S. 88-90; 2014. 1. S. 96-98.

9. Dospehov B. A. Metodika polevogo opyta / B.A. Dospehov. 5-e izd., dop. i pererab. M.: Agropromizdat, 1985. 351 s.

10.Osnovy naukovyh doslidzhen' v agronomii'/ V. O. Jeshhenko, P. G. Kopytko, V. P. Opryshko, P. V. Kostogryz; Za red.

V. O. Jeshhenka. K.: Dija, 2005. 288 s.

11.Kompjuterni metody v sil's'komu gospodarstvi ta biologii' / [Carenko O. M., Zlobin Ju. A., Sklar V. G., Panchenko S. M.] Sumy: Univer. kn., 2000. 203 s.

12.Mojsejchenko V. F. Osnovy naukovyh doslidzhen' v agronomii': pidruchnyk / V. F. Mojsejchenko, V. O. Jeshhenko. K. :

Vyshha shk., 1994. 334 s.

. . . , : (0,75 /) + (2,5 /) 2,74 /, 0,53 / 24,0 %, (0,6 /) + (1,5 /) 2,70 /, 0,50 / 22,7 %. : 37,5 19,1 % ( ), 37,8 19,4 % ( ), .

: , , , , , .

06.04.2015 .

UDC 581:146 MATSKEVYCH V., FILIPOVA L., Cand. of Agricultural Sciences, associate professors Bila Tserkva National Agrarian University vitroplant@i.ua

USING CYTOKININS IN BERRIES CLONAL MICROPROPAGATION

, in vitro Rubus fruticosus L. ( ), Rbes ngrum L. ( ), Rbes rubrum L. ( ), Rbes Grossularia ( ) (0,51,0 /). 2,0 / Matskevych V., Filipova L., 2015.

   

(R. grossularia 69 %). (2,0 /) . Rubus fruticosus L. Rbes Grossularia L.

: , , , , , in vitro.

Problem statement. Using plants clonal micropropagation, particularly in berries, allows solving a lot of problems in plants cultivation: propagation coefficient increase, getting healthy planting material etc. However, this technology needs some improvement depending on the plants species and their varietal characteristics.

Analysis of recent research and publications. Propagation in vitro is possible with direct and indirect morphogenesis. Regenerant plants formation from a callus is the characteristic of indirect morphogenesis.

Direct morphogenesis is characterized by the activation of existing meristem, the formation of buds. Axillary buds growth activation and using axillary sprouts is the most common type of the clonal micropropagation of plants. Plant axillary buds growth in the native conditions is overwhelmed by apical dominance and stimulated by removing the stem top or its processing with cytokinins [1]. only Two types of sprouts are generated with the direct morphogenesis cloning technology (fig. 1).

The first type is characterized with the formation of one sprout with clear-cut dominance.

Engraftments having a part of stem with the leaf and the bud in the axil are formed under such division sprout. The sprout of the future regenerant forms from this bud, adventitious roots form the basal part of the stem. This way of the propagation in aseptic conditions is typical for of potatoes, carnation, chrysanthemum [2, 3].

Figure 1. Exogens cytokinin benzylaminopurine concentration influence on the Rubus fruticosus 'Reubenregenerants development

I, II Different development types of regenerants sprout under direct morphogenesis:

I with the marked apical dominance; with rosettes sprouts formation. 1 benzylaminopurine 0,5 mg/l;2 benzylaminopurine 1,0 mg/l; a regenerant; b regenerat division by cuttings;

the regenerant grown on hydroponics for 15 days is suitable for the planting in the soil.

   

A concentration of 1,0 mg/l is required to activate the growth of axillary buds in Ruben sort blackberry The formed sprouts cluster grows quickly and is further divided into smaller fasciles.

Under low concentrations of cytokinins the regenerants have a developed root system, which make them more suitable morphologically for postaseptic cultivation. Plant organism reaction on cytokinin depended on the plant type. Sprouts with the marked apical dominance were formed in R. fruticosus and R. ngrum (Fig. 2) under the lowest concentration in the experiment (0,5 mg/l).

The quantity of sprouts per one regenerat in these sorts was close to one: R. fruticosus 1,4 and R.

ngrum 1,1. Under the same conditions apical dominance in R. rubrum and R. Grossularia was not observed and they formed respectively 4,9 and 4,7 sprout rosettes. The concentration increase to 1,0 mg /l enlarged the number of sprouts in the rosettes. At the same time, there was a small part of sprouts with vitrification signs. The largest amount of vitrificated plants (47%) was observed in R. Grossularia. In other studied sorts the plants number was 1 - 3%. Further increase in BAP content to 2,0 mg/l enlarged the number of vitrificated plants significantly. The largest amount of vitrificated organisms was observed in R. Grossularia - 69%, and the smallest one - in R. ngrum.




140 .


   

Figure 2. Plants response to exogenous cytokinin (BAP 0,5 mg/l) depending on the plants species:

1. Rbes ngrum. L. apical dominance sprout; 2. Rbes rubrum L. sprouts rosettes.

Under plants cultivating in the culture medium with a high BAP content (2,0 mg/l) the negative impact was amplified. Thus, R. fruticosus regenerantes were of smaller size and formed smaller numbers of sprouts in the rosettes (Fig. 3). Under these conditions R. Grossularia regenerats got chlorotic and died within 10-15 days.

   

Conclusions. Method of rosettes conglomerate formation in basic plants is advisable to use for the propagation of the studied species in vitro. Their inductor of their formation is adding BAP in the amounts of 1.0 2.0 mg/l in the nutrient medium. Higher concentrations are toxic. Increasing the explants donor age reduced the toxicity.

, 12015.

LIST OF REFERENCES

1. .. . - / .. . .: , 2005. 730 .

2. .. г / .. // . . : , 2010. . 3 (74). . 84 87.

3. / . . , .. , . . , . . // ³ . : , 2007. . 46. . 2731.

4. .. in vitro Hosta / . . , . . Գ, .. // . .: 㳿, 2012. . 4. . 7276.

5. .. ̳ / . . , . . . .: , 2005. 267 .

6. http://www.scie.org.au/issue/jber/vol1/no3/In%20Vitro%20Micropropagation%20of%20Blackberry%20(Rubus%20frut icosus.)%20Cultivar%20%E2%80%9CJUMBO%E2%80%9D.pdf

7. file:///C:/Documents%20and%20Settings/User/%D0%9C%D0%BE%D0%B8%20%D0%B4%D0%BE%D0%BA%D1 %83%D0%BC%D0%B5%D0%BD%D1%82%D1%8B/Downloads/SCSB201401V23S01A0015.pdf

8. http://3ismp.bau.edu.jo/abst.pdf

9. http://www.fruit.org.ua/index.php/event/93-ua-kontent/sluzhebnye-stati/244-agrus-biologichni-osoblivosti-agrusu

REFERENCES

1. Kunakh V.A. Botekhnologya lkars'kikh roslin. Genetichn ta fzologo-bokhmchn osnovi / A.V. Kunakh. K.:

Logos, 2005. 730 s.



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2. THE COMPARATIVE STUDY OF ACTIVITY TUBERCULINS Zavgorodniy A.I., Stegniy B.T., Bilushko V.V. National scientific center Institute of experimental and clinical veterinary medicine, Kharkov Purpose to conduct comparative studies in terms of biological activity of commercial batches from different manufacturers tuberculin PPD-mammalian international and national standard samples. Materials and methods. To study the biological activity of a...

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