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636.5.034:579.64 ̲ί ̲Ҳ ֲ .., .., .., .. 㳿 ...

-- [ 1 ] --

5.

Materials and Methods. Intact chickens White leghorn breed was used in the experiment. Chickens

of first and second groups were injected intramuscularly of a plasmid DNA 110 ng dose with liposomalcomplex

of 10 mM phosphatidyl choline CTAB (CTAB 0.5 %). Immunostimulant Cythosol was additionally added

intramuscularly in the second group. The third group and the fourth group were intact.

Chickens of first, second and third groups were infected by control strain JM-UA VCM with infectivity 10000 FUO/ml on 21 th day. We observed chickens during 89 days. Blood serum was collected three times.

The level of nitric oxide metabolites was measured by method Metelskaya V.A. and Gumanova N.G.

(2005).

Results. We observed high levels of nitric oxide metabolites in vaccinated groups and low levels in nonvaccinated chickens.

Conclusions. 1. Reduced of the nitric oxide metabolites concentration in the chickens serum on the background of the Marek's disease development is maintained throughout the study period.

2. There were observed high concentration of nitric oxide in the chickens blood serum at 47-89th day of the experiment, this is due to activation of the immune system and the antioxidant reactions.

3. It seems promising to further, more detailed study of the DNA vaccines impact to the antiradical defense system work in the chickens body.

Keywords: nitric oxide metabolites, DNA vaccine, Marek's disease, poultry, serum.

636.5.034:579.64

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㳿 㳿 , , e-mail: Golembiovska@ukr.net Streptomyces globisporus 7 7Crt . . 7 50+2,5 / , 7Crt 35+2,0 / -.

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.

   

CARRYING CAROTENOID BIOMASS OF STREPTOMYCES IN RATION OF OVIPARUS HENS

Golembiovska S.L., Dvornyc T.V., Lavrenchuk V.Ya., Matselyukh B.P.

Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv It shown productivity of hens oviparous in winter after carrying in their nutrition ration carotenoid biomass strains Streptomyces globisporus 7 and 7Crt. Streptomyces strains deffer from the carotenoid composition.

The strain 7 has formed only one carotenoid - lycopene in number of 50+2,5 mg/L of medium. The strain 7Crt 99, 2014 .



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