WWW.UK.X-PDF.RU

˲ - , ,

 
<< HOME
CONTACTS




( )
.: (050)697-98-00, (067)176-69-25, (063)846-28-10
׸

( )
.: (050)697-98-00, (067)176-69-25, (063)846-28-10
׸
 >>  . C 03.00.00
Pages:   || 2 |

THE COMPARATIVE STUDY OF ACTIVITY TUBERCULINS Zavgorodniy A.I., Stegniy B.T., Bilushko V.V. National scientific center Institute of experimental and clinical veterinary medicine, ...

-- [ 1 ] --

2.

THE COMPARATIVE STUDY OF ACTIVITY TUBERCULINS

Zavgorodniy A.I., Stegniy B.T., Bilushko V.V.

National scientific center Institute of experimental and clinical veterinary medicine, Kharkov

Purpose - to conduct comparative studies in terms of biological activity of commercial batches from

different manufacturers tuberculin PPD-mammalian international and national standard samples.

Materials and methods. To study the biological activity of a comparative study of samples of commercial batches of purified tuberculin (PPD) for mammals produced GF "Sums biofactory" FGUF "biofactory Kursk" international standard sample NIBSCE (United Kingdoom) and National standard national sample dried purified tuberculin PPD-mammalian.

The experiment was carried out on 10 clinically healthy bullock 10-12 months of age, body weight 230kg.

Activity series tuberculin different manufacturers were studied in 3 dilutions: 200, 1000 and 5000 IU.

Allergens were injected intradermally in depilovani skin in the middle third of the neck using injector "B-7" at doses of 200, 1000 and 5000 IU in a volume of 0.1 cm. Accounting allergic reactions in animals was performed 72 hours after administration of allergens.

Results. Of average size in vnutrishoshkirnyh allergic reactions in cattle to enter tuberculin in a dosedependent administration of gradually increasing the minimum (4,10,37) mm - the introduction of tuberculin in a dose of 200 IU to a maximum of (22,22,01) mm - 5,000 IU. The obtained results naturally reflect a gradual increase in the intensity of allergic reactions, depending on the increase in the dose of tuberculin. For example, after the introduction of tuberculin series number 24 (Sums biofactory) at a dose of 200 IU noted potovshennya folds of skin in animals on average (4,10,4) mm; 1,000 IU - (6,20,60) mm and 5000 IU - (18,01,65) mm.

Thus, the total intensity of allergic reactions after administration of the drug at doses of 200 and 500 IU was less on research series number 24 than control NIBSE.

Conclusion. The research found that PPD-tuberculin mammals of SE "Sums biofactory" FSUE "Kursk biofactory", NIBSCE and national standards for mammalian tuberculin (Ukraine) for biological activity are equivalent diagnostic agents for in vivo diagnosis of tuberculosis in allergic animals.

Prospects for future research are to develop national standard samples of purified allergen dry with atypical mycobacteria (AAM), which would be responsible for diagnostic as the national standard for mammalian tuberculin when used in simultaneous allergy test to determine the nature of allergic reactions to tuberculin in cattle.

Keywords: tuberculin (PPD) for mammals, biological activity, allergic reactions, international units, cattle.

636.09:619:616.988.27

Ҳ ԲҲ IJί -

Բֲ ² ̒ί .., .., ..

- 㳿 , . , e-mail:vetbiotk@i.ua . , - -.

: , , -, , , , (canine distemper virus, CDV). , . ³ Morbillivirus Paramyxovirinae Paramixoviridae. ѳ Paramixoviridae Bornaviridae, Filoviridae Rhabdoviridae Mononegavirales. Paramixoviridae [1] Paramixoviridae ( Morbillivirus, Paramyxovirus, Rubulavirus) Pneumovirinae ( Pneumovirus) [2].

: (measles virus MV), (RPV), (Pestedes-petits ruminants Virus, PPRV), ( phocine distemper virus, PDV); porpoise morbillivirus, PMV DMV [3].

98, 2014 .

, ' ﳿ, , , .

, , . [4], , , . [5]. . , , - , ().

[6, 7].

: - .

. Canine Distemper Virus (CDV) GenBank, EMBL ( - ), DDBJ ( ), PDB sequences.

: highly conserved region of the NP gene of the Ond-CDV strain consensus sequence of 55 gene H, , highly conserved region of the NP gene (GenBank: AJ009656) CDV.

conserved region of the nucleocapsid protein N gene CDV , , . ' "Vector NTI" PerlPrimer , CDV F5 ( ) CDV R6 ( ) ( BLAST) . 㳿 , .

㳿 (, . ). 25 : 67 -Ѳ ( 8,8), 16,6 m (NH4)2SO4, 2,0 gCI2, 0.01 % -20, 100 , , , , 50 , 2 . q-, 5 . 30 볿.

35 . 95 45 , 58 30 , 74 40 ( 5 ). 1,5 % ( ) - 䳺 10 /. - ( ) - . () .




140 .


. - Canine Distemper Virus (CDV) ( ) . , ' . (http://www.premierbiosoft.com/tech_notes/PCR_Primer_Design.html):

' ;

' ;

;

.

3 , ( ) CDV F1 CDV R2 [8]; CDV F3 CDV R4 [9] CDV F5 CDV R6 ( 1). ˳ ( , . ).

   

55 60 ( 1).

CDV F5 CDV R6. 58 .

. 1. (.. ) (1 (GeneRuler 50 bp DNA Ladder), 2 5 CDV F1 CDV R2;

   

( ).

( , ) - CITO TEST CDV Ag. 2.

   

. .

58 .

- 䳺 , 1,7 10 TCID50 Virus febris contagiosae canis.

- - ( , ) - CITO TEST CDV Ag , , -.

- , - .

1. Pringle, C. R. Virus taxonomy at the XIth International Congress of Virology, Sydney, Australia, 1999. - Archives of Virology - Volume 144, Issue 10, - p.



Pages:   || 2 |
 >>  . C 03.00.00
:

631.52:631.523:633.522 , , , , , (. ) (. ) Ͳ IJ òֲ ͪ ֲ . ...

̲Ͳ ² ֲ Ͳ ò .. () 13 2013 . ò Ҳ Ͳ ֲ 6.051701 㳿 , 㳿 ...

See discussions, stats, and author profiles for this publication at: http://www.researchgate.net/publication/276864502 ֲ IJ ί Ҳ . (. . , , ί Ҳ). TECHNICAL REPORT APRIL 2007 DOI: 10.13140/RG.2.1.1544.0805 DOWNLOADS VIEWS 7 AUTHORS, INCLUDING: Andrey Kovalchuk Natalia Kovalchuk Uzhhorod National University Uzhhorod National University...

ֲ Ͳ :635.5+634.1 òͲ Ҳ ֲ PHYTOPHTHORA INFESTANS (MONT.) DE BARY ̲ ˲ 06.01.11- ȯ 2004 , ˳ , ,...

633.63:631.527.531.12 ˲ , , . , .-. , . . ., . . . - , .-. (. ) ֲ ʲ Ͳ ...

ISSN 2076-5835. ³ . 2 (255) : 616.981.717-036.21:576.895.421:591.555/556.572.1/4:577.4(477.41) .. в ʲ Dz ʲ ˲ Dz ȯί Ҳ - - , ...

ISSN 2079-8334. 㳿. 2014. 1(43) 616.438-091.8-003.9:616-001.17:616-092.4 . . , . . , . . , . . ³ . .. , . ³, . .. , . вί Dz ò Ͳ Ͳ ̲ Ͳ βί ϲ² ...

37.033 Ͳֲ ֲί ϲ ò Ͳ .., .. 㳿 . , ...

˲Ѳ ˲˲ֲ : IJ, 2008. . 114 630*416:630*181 . . ̲, . . * ’ ̲ в ò . .. IJ , , , , ....

̲ 504:637.12 ֲ ò ʲ ( IJ òͲ ) .., .. , , - . ...




( )
.: (050)697-98-00, (067)176-69-25, (063)846-28-10
׸


 
<<     |    
2013 www.uk.x-pdf.ru -