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̲Ҳ ʲ Dz ʲ ...

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S. kanamyceticus . S. kanamyceticus 1, dgr1, 2--D-, , 1375. 1 , 1375. , , dgr1, , 1, . ֳ S. kanamyceticus (.8) S. kanamyceticus . S. kanamyceticus . 6 60 ST STM 0,1-1% , 2% R2YE . 0,410-4 6,710-3. : 1) 781met10 cys2 kanB781 92 ade1 eryR1 (232 , 10 ); 2) 781met10 cys2 kanB781 21ilv1 genR1 (241 , 11 ); 3) 131his1 tyr1 kanD131 4thr1 rifR1 (176 , 10 ). . kan ery+ (r A =0,75), kan rif+ (r A =0,80), kan gen+ (r A =0,70). kanB781 kanD131, , , .

S. kanamyceticus 1375 ( ), 1 ( ) 5 an- . , . г - 1. S. kanamyceticus , .

10-250 / S. kanamyceticus . S. kanamyceticus .

, 1375.

- S. kanamyceticus 1, . 115 , - (KanR), 105 - (NeoR), 100

- (GenR) 90 - - (AprR) . , , . GenR- . 1 . GenR-, NeoR-, AprR- , 1, . GenR-, - , (108,2% 1), (60,6%) , , 1, ""- (9,5%).

- 1 .

- S. kanamyceticus 1 GenR - genR10 pIJ702 S. lividans 66. S.

lividans : ) pIJK12, 1; ) pIJG31 - genR10. , , kmrB, 4,2 6,1 ..., (.9). kmrB , , (.2) - - S. kanamyceticus 1. kmrB , 831 .

. ( ATG, TGA). RBS (GAGGA), 3- 16S S.lividans. G+C , , , 70, 52 92%. kmrB 16S , 㳿 . () kmrB , 㳿 16S S. tenebrarius ( - 53,9%, - 77,9%). - 16S - . - kmrB S.

kanamyceticus , kmrB, genR10, genR8, genR8.1. г . kmr Kan-- - kn12 (.10). , S. kanamyceticus kmrB. ij , , S. fradiae Micromonospora zionensis.

5 S. kanamyceticus -. S. kanamyceticus -. 16 AseI- 12 DraI- 1, 40 1800 ... S. kanamyceticus 7715 8217 .... kan12, genR8 genR10.

1 kan12 AseI- D (98 ...) J (220 ...), J(297) K(450 ...). genR10 genR8 (420 ...), ' '. kan12, genR10 genR8 (8217 7818 ..., ), 1 (7788 ...), kmrB. ֳ S. kanamyceticus, .

   

: Km -, Gm-, Tm-, Sn-, Nm-, Ap-, Sm-, Pm-.

S. kanamyceticus 1 pIJ702, pWHM4, pTO1 pSET152 . (pSOK101, pSOK201 pCHZ101) (pSET152 pVWB) S. kanamyceticus E. coli ET12567(pUB037). , 2,210-5 2,510-4. pSET152 ( attP- 31) pVWB ( attP- VWB) 100%. 20% . pSET152+. pVWB , . pSET152+- PstI- pSET152 (800 ..) , S. kanamyceticus (attB1 attB2). S. kanamyceticus, . 4 ORF, ORF1, , , pSET152. S. kanamyceticus pSET152 S. kanamyceticus.




140 .


S. kanamyceticus - , - , , 2- .

Sacch.

erythraea, S. ambofaciens, S. peucetius subsp.caesius, S. kanamyceticus. , . , . .., ᒺ, .

:

1. - , . . , , , , , .

2. . S. coelicolor A3(2) S. ambofaciens , .

3. Sacch. erythraea, , , , S. peucetius subsp.aesius, , S. kanamyceticus, . , , , .



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. . , , , , . Grounded the necessity of search of fuels from refurbishable sources, able to replace oil diesel. The estimation of vegetable butter and biotdiesel as alternatives is conducted to the oil fuel-oil. Methoxymethane, vegetable butter, biotdiesel, methyl ether, blenderized fuels. 631.315:629.783...

㳿, 3 2011 REVIEW OF BASIC WORLD PROCESSES AND TENDENCIES IN THE FIELD OF , COUNTERACTION THE THREATS OF NUCLEAR DISTRIBUTION, NUCLEAR TERRORISM AND POWER SAFETY .., .., .. Voronenko V.V., Skaleckiy Yu.M., Torbin V.F. ...

㳿, 1 2013 animals allowed to set the parameters of the norm of polarization properties of the thyroid and suprarenal . glands tissues in rats. . Prospects of further researches. Perspective in this direction is a study of...

ֲ ̲ ̲ò ²ò . .. ² ˲ ²в 579.841.11.22 Ͳ òί Ҳ ˲˲IJ RALSTONIA SOLANACEARUM (Smith, 1896) ² 03.00.07 ȯ 2002 . 쳿 㳿 㳿 ....

1 (56) : 2012 innovative technologies in agriculture is the key to highly efficient and profitable process of production. The development of the domestic crop is by bringing into circulation of agriculture of the new plants, equipments, technologies, economy of labor and energy cost, and its increase quality and competitive of products in the world market. 60:167.2 (477/9) ˲ ˲ Ҳ ò ...

̲Ͳ ² ֲ Ͳ ò ò Ͳ ֲ 6.051401 2013 㳿. . . . . . . . 6.051401 . . . / .: . . .: , 2013. 26 . : .., ..., :...

IJ ղ ϲ òί Ҳ ˲ ˲ .. , . , , . 11 ( , ) . 10-11 ( ), ...

ֲ ̲ ̲ò ²ò . .. ' I : 576.8.077:576.835.11/636.4 I I II 03.00.06 2001 㳿 iii i : ...




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