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糿 - Bacillus mycoides HB2 Streptomyces albus CEST113.

() : [ ., 1997], [Weber et al., 1985], [Richardson et al., 1990], [Segura et al., 1997]. , () [Henkel et al., 1990; ., 1999].

[Dickens et al.,1996].

[Lowry, 1951].

ij .

(), (), N--N-, N--N--N- N--N-- (), 5,8- [Hopwood et al., 1985; ., 1990; ., 1995].

[Hopwood et al., 1985; Kieser et al., 2000], [Weber et al., 1985]. , [, , 1980; ., 1990, ., 1990]. , 2--D-, [Hodgson, 1982].

, [Hopwood et al., 1985; Kieser et al., 2000] , [Hopwood et al., 1985; Kieser et al., 2000].

E. coli Streptomyces , [ ., 2000].

[Hopwood et al., 1985;

Kieser et al., 2000].

E. coli [Sambrook et al., 1999].

- ( , , , 4-- - 4, - , (), [Hopwood et al., 1985; Kieser et al., 2000; Sambrook et al., 1999]. - S. kanamyceticus , [ .,1998]. BLAST 2.0, CODONPREFERENCE, REFLECTION, NEBCUTTER, TRANSLATE, PILEUP BOXSHADE CLUSTAL W [Altschul et al.,1997].

STATGRAFIC icrosoft Excel 2000. , [ ., 1978; , 1980].

˲

1. . 16 - (S. coelicolor A3(2), S. lividans 66), (Sacch. erythraea NRLL2338, S.

ambofaciens ATCC23877, S. fradiae B-45, S. antibioticus OL71), (S. peucetius subsp.caesius 27952, S. peucetius ATCC29050, S. nogalater IMET43360 S. galilaeus KI022), (S. globisporus 1912, S. cyanogenus S136 S. fradiae Tu2717), (S. rimosus 183, S. aureofaciens 019(8) (S. caespitosus IMET43411). , .

.

, . - , . , , . ( 40 100 ) ( 10 20 ). (, Sach.

erythraea), (, S. peucetius subsp.caesius) (.1). , , , , , ( , ). .

, S. coelicolor A3(2) S. ambofaciens , - (CmlR). . (-, CmlS) CmlSRmnS ( ) . CmlS- CmlSRmnS- . (CmlR*-). CmlS- S. coelicolor A3(2) ( 5-7%). CmlSRmnS CmlR*-. , . () - S. coelicolor A3(2). , [Coyne et al.,1984; Hromic, Kirby, 1989] CmlS-. ֳ , - . Cml - S.ambofaciens.

R CmlR* - S. coelicolor A3(2) ( cmlR103, .2) , : AUD-ScI ( 15 ... 50 ) AUD-ScII ( 20 ... 40 ). ³ 2,8 25 ... S.ambofaciens. ֳ , .

Sacch.

erythraea, , , . . , (.3). , , , rif-, cml-, tsr- str- . , rif- cml : rifR5 rifR8 - ura1 phe1, cmlR45 ura1 met1. Sacch.erythraea pSE201. , . RifR, , 10 / . ( X ) (126,75,9% Sacch.




140 .


erythraea 5) (11,3%) - , X +2. 5, StrR- ( 22,7%) . ( 12%) ""- StrR-. 57% CmlR ( 20-40 / ) , . (140,68,9%) - (20,0%) TsrR , 2,5 / (.1).

, RifR-, CmlR-, TsrR- StrR- Sacch. erythraea. . , , , , , Sacch. erythraea 5. CmlR-, TsrR- StrR . . RifR-, CmlR-, TsrR- StrR .

S.

peucetius subsp.aesius, . , , , . ,

   

(DnrR) i (DxrR), S. peucetius subsp.aesius.

, DnrR- , DxrR- .

2. S. globisporus 1912.

S. globisporus 1912 BamHI. Gem11, in vitro , E.coli KW251.



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ֲ ̲ ̲ò ²ò . .. ² ˲ ²в 579.841.11.22 Ͳ òί Ҳ ˲˲IJ RALSTONIA SOLANACEARUM (Smith, 1896) ² 03.00.07 ȯ 2002 . 쳿 㳿 㳿 ....

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See discussions, stats, and author profiles for this publication at: http://www.researchgate.net/publication/276864502 ֲ IJ ί Ҳ . (. . , , ί Ҳ). TECHNICAL REPORT APRIL 2007 DOI: 10.13140/RG.2.1.1544.0805 DOWNLOADS VIEWS 7 AUTHORS, INCLUDING: Andrey Kovalchuk Natalia Kovalchuk Uzhhorod National University Uzhhorod National University...

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