WWW.UK.X-PDF.RU

˲ - , ,

 
<< HOME
CONTACTS




( )
.: (050)697-98-00, (067)176-69-25, (063)846-28-10
׸

( )
.: (050)697-98-00, (067)176-69-25, (063)846-28-10
׸
 >>  . C 03.00.00
Pages:     | 1 |   ...   | 3 | 4 ||

˲˲IJ RALSTONIA SOLANACEARUM (Smith, 1896) ² ...

-- [ 5 ] --

Lipopolysaccharides (LPS) of 8 strains of Ralstonia solanacearum (767, 4157, 5712, 7944, 7945, 7955, 8089 and 8110) characterized by different types of structures of O-specific polysaccharide chains have been investigated. Rhamnose was the predominant monosaccharide of LPS of 7 strains tested while R. solanacearum 4157 LPS was characterized by high content of arabinose. Xylose was also observed in LPS of a number R. solanacearum strains. Analysis of fatty acids gave a possibility to divide R. solanacearum strains tested into two groups: the first group was represented by strains which LPS contain hydroxy-fatty acids with 12-18 carbon atoms in their chain, and the second one was characterized by shorter length of chain (C10-C14).

Using methods of ring-precipitation, agglutination, double immunodiffusion by Ouchterlony, immunoelectrophoresis and rocket immunoelectrophoresis, ELISA and ELISA-inhibition the R. solanacearum strains investigated were divided into five serogroups on the basis of LPS specificity.

The results of SDS-PAGE indicate LPS of all investigated strains have a typical for S-LPS bimodal distribution and include two main types of molecules: high-molecular mass S-fraction and low-molecular mass R-fraction of LPS. It was shown that LPS one and the same serogroup are characterized by similar electrophoretic distribution, while in compare with others serogroups electrophoretic distribution was strictly individual.

Immunoblotting analysis indicated the zones of serological activity of LPS with respect to homological and heterological antiserum. It was shown the expected arrangement of common antigenic determinants in S-, SR- and R-zones of LPS.

On the basis of LPS pyrogenicity and toxicity (LD50) R. solanacearum strains were divided into two groups: more pyrogenic and toxic (5712, 7945, 7955 and 8110 - LD50 8-12 mg/mouse) and less pyrogenic and toxic (767, 7944, 8089 and 4157 - LD50 30-40 mg/mouse).

UV-impact of LPS solutions showed the antigenic activity of LPS correlated with time of irradiation: the increase of irradiation exposition time leads to decrease of serological specificity of LPS to O-antiserum.

Modification of lipid A by O-deacylation, N-,O- deacylation and dephosphorylation causes the complete loss of their toxicity and pyrogenicity.

Key words: lipopolysaccharide, serogroups, heterogeneity, pyrogeneity, toxicity, modified LPS, Ralstonia solanacearum.



Pages:     | 1 |   ...   | 3 | 4 ||
 >>  . C 03.00.00
:

㳿, 3 2011 REVIEW OF BASIC WORLD PROCESSES AND TENDENCIES IN THE FIELD OF , COUNTERACTION THE THREATS OF NUCLEAR DISTRIBUTION, NUCLEAR TERRORISM AND POWER SAFETY .., .., .. Voronenko V.V., Skaleckiy Yu.M., Torbin V.F. ...

1 (56) : 2012 innovative technologies in agriculture is the key to highly efficient and profitable process of production. The development of the domestic crop is by bringing into circulation of agriculture of the new plants, equipments, technologies, economy of labor and energy cost, and its increase quality and competitive of products in the world market. 60:167.2 (477/9) ˲ ˲ Ҳ ò ...

ISSN 2079-8334. 㳿. 2014. 1(43) 616.438-091.8-003.9:616-001.17:616-092.4 . . , . . , . . , . . ³ . .. , . ³, . .. , . вί Dz ò Ͳ Ͳ ̲ Ͳ βί ϲ² ...

̲Ͳ ² ֲ Ͳ ò .. () 13 2013 . ò Ҳ Ͳ ֲ 6.051701 㳿 , 㳿 ...




( )
.: (050)697-98-00, (067)176-69-25, (063)846-28-10
׸


 
<<     |    
2013 www.uk.x-pdf.ru -