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˲˲IJ RALSTONIA SOLANACEARUM (Smith, 1896) ² ...

-- [ 2 ] --

. () ' ̳ (, 2000); 6th Conference of the International Endotoxin Society (Paris, 2000); (, 2000); ̳ - (, 2001); the European Materials Research Society (E-MRS 2001) Spring Meeting (Strasbourg, 2001); XVI International Symposium on Glycoconjugates GLYCO XVI (Hague, 2001); 11th European Carbohydrate Symposium Eurocarb-11 (Lisboa, 2001); , 135- . . (, 2001); 㳿, 쳿, 㳿 㳿 . (, 2001).

. 8 : 4 , 3 , 4 .

. 154 , , , , , , , 219 ( 194 ). 17 20 .

IJ 1. ˲ , R. solanacearum, 600 , , , .

   

8110 4 * - - . ' 1% .

(25000?g, 40 .) . - G-50 0,025 - , 4,5. Dubois (1956), (1958), Lowry (1951). [Davies, 1957], 2--3--D- () [Droge, 1970]. Chrom-5 (). Hitachi (). Hewlett Packard 5890 () - 5970 (Hewlett Packard, ) '.

, , , , 糿 , , ELISA ELISA-, , , , - , R. solanacearum, .

- Laemmli (1970) 5% 12% 30 mA. [Tsai, 1982].

Pyle (1985) (Schleicher-Schuell, 0,45 ) 150 mA 4 . 22 3,3?-.

ϳ , (0,01-0,005 /), 3 . , (0,5 ). , 3- 1,4; 2,2 .

(50) , , (50-300 /). 10 ;

(10 ) 0,9% NaCl.

- ( 0,2 N N 99% , 18 ., 50?), N-,O- ( , 40 ., 100?) ( 40% HF, 24 ., ). ( ) .

- (-101, ). 350-600 , . 2 5 7-10 . 糺 .

, '.

IJ 3. IJ R. solanacearum,

8 R. solanacearum. , : , , : 2,7-7,8%. R. solanacearum, 144000?g, 4 ., . : 16,5-47,25% , 1,44-3,6% , 0,25-3,25% .

, , . 4157, (26,53-92,8%). . 4157 (84,6%) (13,3%). , (7945, 5712 767) , R. solanacearum (61,36%, 8,72% 6,81%, ).

: , , , . (1,4-16,8%) (0,66-8,48%). (0,25-2,08%) (0,03-0,4%).

. : ( ), (0,2-6,0%) (0,11-0,8%).

. 5712 .

[- ( ) - ( )] , S- .

, (. 2).

³, , R.



140 .


solanacearum. R. solanacearum 3- (33,53%), (19,22%), 2- (21,55%), 2- (3,12%) (2,66%) . . 7945, 7955 8110, . , , . , . 7945, 7955 8110 16:0 (12,56%, 6,09% 7,29%, ); .7945 2--16:0 (2,67%); . 7945, 7955 8110 3--15:0 (1,38, 1,35 2,66%, ); . 7945 8110 -9-18:0. , 2--18:1 (8,3%).

ֳ , 3--14:0 Burkholderia cepacia [, 1989] Burkholderia, R. solanacearum. Erwinia carotovora, Enterobacteriaceae [, , 1991; , 2002; Branderburg et al., 1993] 3--14:0, 16:0 14:0 , R. solanacearum .

2 R. solanacearum ( % )

   

767, 7944, 8089 4157 . 7945, 7955 8110. : 2--11:0 (. 4157-6,61%), 3--10:0 (. 8089 4157 18,23 3,45%, ), 3--11:0 (7944, 8089 4157 3,0%, 1,14% 1,15%, ), 2--12:0 (8089 4157 23,6 4,34%, ), 3--12:0 (7944, 8089 4157 25,64%, 18,12% 24,47%, ), 2--13:0 3--13:0 (. 4157 14,1% 6,15%, ), 3--14:0 12:0 (7944, 8089 4157). 3--10:0, 3--12:0 2--12:0 P. syringae P. fluorescens [, , 2000].

. (1993) 25 R. solanacearum 8 - (. 3).

: 1) - ?-(12)-, -(13)- ? ?-(13)-' N-; 2) - , , L- L- ; 3) - , , , -; 4) 8089 4157, , - , .

   



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